Marta Calatayud Arroyo
Center for Microbial Ecology and Technology, Belgium
Title: IN VITRO ASSESSMENT OF ARSENIC AND GUT MICRO BIOME INTERPLAY
Biography
Biography: Marta Calatayud Arroyo
Abstract
Microbe-host cross-talk is a key factor in human health and while the gut barrier controls (micro-) nutrient absorption, it also fends off antigens or xenobiotics. One of the contaminants of highest health concern is arsenic, which affects more than 100 million people worldwide, causing cancer, cardiovascular and metabolic diseases. Human exposure to inorganic and organic arsenic is largely caused by the ingestion of contaminated drinking water and foodstuffs, mainly rice and seafood. Although most of the arsenic is absorbed in the small intestine, significant amounts could reach distal segments of the gut. Specifically, at the colon, a mucus layer is covering the epithelial surface, protecting the colonocytes from the luminal milieu. This specific niche, due to its close contact with epithelial cells, is gaining more attention in host-microbe interaction studies. From our results, gut microbiome was affected by arsenic in the simulator of the human intestinal microbial ecosystem (SHIME®). 7 human fecal samples were stabilized in the SHIME reactor and exposed to environmentally relevant levels of arsenic (0.01 or 0.1 mg/L) for 7 days. Gut microbiome and specifically mucus associated microbiota reduced its metabolic activity, represented by a decrease in short chain fatty acids and ammonium production (48-89% of reduction compared to the control). In addition, the microbial structure in the mucus niche was specifically affected, increasing the richness and reducing the evenness in the community. These results support the hypothesis of considering the mucus ecosystem in the gut as sensitive “target organ” of arsenic toxicity.